Run the game, leave it open in the background and change back to the editor window. In the scene tree dock, you should now see two new buttons: “Remote” and “Local”. “Local” is the regular scene tree that you set up in the editor. “Remote” is the scene tree as it looks right now, in the running game. That is, if you add or remove nodes at runtime or modify any properties, those changes should show up here. So click on “Remote” and then unfold the tree until you find your AnimatedSprite, select it and check the inspector to see if the visible property is set or not.